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Osteogenic characterization of D1 stem cells on tissue culture plastic. DNA content (A), alkaline phosphatase activity (B), and osteocalcin (C) of D1 cell lysates over time when cultured in tissue culture plastic in Dulbecco's modified Eagle medium alone (open circle) or with osteogenic supplements (solid circle) (50 μg/mL ascorbic acid, 10 mM β-glycerophosphate). *p < 0.05 between noted conditions (medium vs osteogenic supplements) at the same time-point.
