FIG. 3.

Osteogenic characterization of primary human bone marrow stromal cells (hBMSCs) on tissue culture plastic. DNA content (A), alkaline phosphatase activity (B), and osteocalcin (C) of primary hBMSC lysates over time when cultured in tissue culture plastic in alpha minimum essential medium alone (open circle, hatched bar) or medium with osteogenic supplements (solid circle, filled bar) (50 μg/mL ascorbic acid, 10 mM β-glycerophosphate, and 0.1μm dexamethasone). Values represent means and standard deviations (n = 4). ^*p < 0.05 between noted conditions (medium vs osteogenic supplements) at the same time-point.