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Increases of relative luciferase activity (shown on the Y-axis) were used as the readout of N-C interaction. pRLTK, Renilla luciferase activity for adjustment of transfection efficiency; p5-GAL-LUC, N-C interaction driven luciferase reporter; GAL-ARHLBD and VP16-ARN, components of the two-hybrid system for N-C interaction; pSG5hAR, wild-type AR plasmid. *P<0.05 compared to the control.
