Table 1.
Primers and probes used in real-time RT-PCR assays for CAL serogroup viruses and CVV and for internal control detection
| Name of primer or probe | Sequence (5′→3′) | Nucleotide start | Reference |
|---|---|---|---|
| CE-NC-F1 | GTGTTTTATGATGTCGCATCA | 94 | This study |
| CE-NC-F2 | GTTTTCTATGATGATGCATCC | 94 | This study |
| CE-NC-R1 | CATATACCCTGCATCAGGATCAA | 153 | This study |
| CE-NC-R2 | CACAAACCCTGCATCTGGATCAA | 153 | This study |
| CE-NC-FamMGB | Fam-CAGGTGCAAATGGA-MGB | 116 | This study |
| CV-Mex-F | GCACTCTGGCAGGCAGGA | 2220 | This study |
| CV-Mex-R | GACGTCTGTTAAGAAGCAAGTTGAGTTT | 2520 | This study |
| CV-G1-F | CCAATGCAATTCAGGGCAGT | 2246 | This study |
| CV-G1-R | TGAGTCACCACATGCTGTAAGGT | 2358 | This study |
| CV-G1-VicMGB | Vic-AAGAATGCCATAATGCA-MGB | 2273 | This study |
| GFP forward primer | CACCCTCTCCACTGACAGAAAAT | 549 | Tavakoli et al. (2007) |
| GFP reverse primer | TTTCACTGGAGTTGTCCCAATTC | 470 | Tavakoli et al. (2007) |
| GFP probe | 6-FAM-TGTGCCCATTAACATCACCATCTAATTCAACA-TAMRA | 525 | Tavakoli et al. (2007) |
LACV sequence is from GenBank accession K00610 (LACV S segment). Nucleotide numbers are given based on the K00610 accession, but CE-NC-F2 and CE-NC-R2 do not perfectly match the sequence of the isolate represented by this accession. CVV sequence is from GenBank accession AF186243 (CVV strain 807270 M segment). Nucleotide numbers are given based on the AF186243 accession, but the CV-Mex-R primer does not perfectly match the sequence of strain 807270. GFP sequence is from GenBank accession EU341596 (cloning vector pGFPm-T).