a) Reduction of phosphorylated p38 MAPK activity in cartilage of DNp38 transgenic mice (+/−) in comparison to that of WT mice (−/−) at 53 weeks of age. Western blot analysis of mouse cartilage extract was performed with an antibody against phosphorylated p38 MAPK (p-p38) and an antibody against p38 protein (p38), respectively. Equal amount of protein was loaded in each lane. Densitometry quantification of the protein bands was shown (error bars indicated standard deviation).
b) Reduction of phosphorylated p38 MAPK activity in growth plate cartilage of DNp38 transgenic mice (+/−) in comparison to that of WT mice (−/−) at birth. Immunohistochemical analysis of mouse growth plate cartilage was performed using an antibody against phosphorylated p38 MAPK (p-p38) and an antibody against p38 protein (p38), respectively.