Figure 12.

HILIC/CEX of a semipurified synthetic amphipathic α-helical peptide (N). Column: same as Fig. 3. Conditions: linear AB gradient (2.5 mM NaClO₄/min, following 10-min isocratic elution with 10% eluent B) at a flow-rate of 1 mL/min, where eluent A is 10 mM aq. TEAP, pH 6.5, and eluent B is 10 mM aq. TEAP containing 350 mM NaClO₄, pH 6.5, both eluents also containing 65% v/v ACN; temperature 25°C. Peaks 2 and 3 represent acetylated peptide impurities; peak 4 represents a Lys-deletion peptide. The sequence of peptide N is shown in Fig. 13. Reprinted from ref. [43] with permission from Munksgaard.