Resolution of pooled chicken erythrocyte H1 variants by HILIC/CEX. Columns: (A) RPC, Chromegabond MC-18 (250 × 4.6 mm id, 5 μm particle size, 300 Å pore size); (B) HILIC/CEX, same as Fig. 19. Conditions: (A) RPC, linear AB gradient of 5−60% v/v ACN in 0.1% aq. TFA over 120 min at a flow-rate of 0.8 mL/min; (B) HILIC/CEX, linear AB gradient of 380−590 mM NaClO4 in 10 mM aq. propionic acid, pH 6.5, containing 70% v/v ACN over 240 min at a flow-rate of 0.8 mL/min. Reprinted from ref. [58] with permission of Academic Press.