Table 1.

Absorption Maxima and Photocycle Lifetimes of AppA and Its Various Clones and Mutants

mutant	flavin absorbance max dark (light) ± 0.5 nm	photocycle τ (s)
AppA1–126	446 nm (458 nm)	948.5 ±6.0
AppA17–133	446 nm (458 nm)	690.9 ±3.1
AppA-full	443 nm (457 nm)	795.0 ±2.3
AppA W302F	445 nm (458 nm)	786.2 ±5.1
AppA1–126 W64F	446 nm (458 nm)	776.3 ±5.4
AppA17–133 W64F	446 nm (458 nm)	477.3 ±2.0
AppA1–126 W104A	443 nm (450 nm)	6.0 ±0.16 (58%), 18.0 ±0.7 (42%)
AppA-full W104A	443 nm (443 nm)	nda
AppA1–126 M106A	447 nm (458 nm)	643.2 ±6.0
AppA-full M106A	444.5 nm (458 nm)	473.9 ±4.6
W104M-M106W in AppA1–126	445 nm (nd)a	11.6 ±0.1 (20%), 50.8 ±0.1 (80%)

^and = not determined.