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rHC was a safe DDV component to delivery antidotes. Primary cultures of mouse spinal cord were exposed to BoNT/A (■) or rHC (○) respectively in indicated concentrations for 16 hrs. Potassium-evoked glycine release was measured. Results expressed as percentage glycine release compared with untreated control. Data points are the mean (± SD) of three separate experiments each determined in triplicate.
