Table 5.

Antibodies used in the study.

Protein	Primary ab, company and order number	Type and dilution	Inc temp + time	Blocking	Biotinylated 2 nd Ab all diluted 1:200	Buffer	Inc time in RT
PRAB	Zymed Lab Inc, 18-0172	Mc mouse anti human 1:150	RT 60 min	1.5% NHS	horse anti-mouse	1.5% NHS in PBS	45 min
PRA	Novocastra Labs Ltd, NCL-PGR312	Mc mouse anti human 1:500	RT 60 min	1.5% NHS	horse anti-mouse	1.5% NHS in PBS	45 min
PRB	Affinity BioReagents Inc, MA1-411	Mc mouse Anti chick 1:100	RT 60 min	1.5% NHS	horse anti-mouse	1.5% NHS in PBS	45 min
AR	DakoCyto-mation Inc, M3562	Mc mouse anti human 1:100	+4°C o/n	2% NHS	horse anti-mouse	2% NHS in PBS	30 min
GR	Affinity Bio Reagents Inc, PA1-511A	Pc rabbit anti human 1:1000	+4°C o/n	2% NGS	goat anti rabbit	2% NGS in PBS	60 min
COX-1	Santa Cruz Biotechnology Inc, sc 1752	Pc goat anti human 1:100	+37°C 60 min	10% NRS	rabbit anti goat	10% NRS in PBS	30 min
COX-2	Santa Cruz Biotechnology Inc, sc 1745	Pc goat anti human 1:100	+37°C 60 min	10% NRS	rabbit anti goat	10% NRS in PBS	30 min

Mc = monoclonal, Pc = polyclonal

RT = room temperature

biotinylated horse anti-mouse (Vector, Cat# BA-2000)

biotinylated goat anti rabbit (Vector, Cat# BA-1000)

biotinylated rabbit anti goat (Vector, Cat# BA-5000)