Table 1.

CREB HaloCHIP-chip positive and negative predictive values.

Positive category	Enriched promoters	PPV
Top 1%	12 of 12	100%
Top 5%	11 of 12	92%
Top 10%	6 of 12	50%
Negative category	Enriched promoters	NPV
Bottom 50%	2 of 26	94.50%

Twelve promoters each from the Top 1%, Top 5%, and Top 10% categories of the HaloCHIP-chip data were chosen to determine the positive predictive value and 36 promoters were chosen from the Bottom 50% of the list for determination of the negative predictive value. SYBR green qPCR was used to determine the presence of these promoters in triplicates samples of HT-CREB experimental and untransfected control HaloCHIP DNA and promoters were considered enriched if having a signal:background ratio ≥ 2-fold between the experimental and control sample. Based upon these results, further analysis of the HaloCHIP-chip data was limited to promoters identified within the Top 5% of the list.