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. 2009 Jun;18(6):1155–1164. doi: 10.1002/pro.120

Table I.

Kinetic and Thermodynamic Parameters Obtained from Limited Proteolysis of WT and TM Iso-1-Cytochromes c at 22 ± 1°C and pH 7.0

Variant
WTa TMb
(kcat/KM) × Kop′ (M−1 s−1)c 1000 ± 500 6600 ± 500
Kop′d 0.0017 ± 0.0011 0.011 ± 0.004
Inline graphic (kcal mol−1)e 3.8 ± 0.4 2.6 ± 0.3
a

Data from three independent trials.

b

Data from two independent trials.

c

Error in (kcat/KM) × Kop′ is taken as the larger of the standard deviation of the two (TM) or three (WT) independent trials or the average of the standard errors reported by SigmaPlot for the slopes of plots of kobs versus proteinase K concentration in the individual trials.

d

kcat/KM = 6 × 105M−1 s−1 was used to calculate Kop′. Reported error is from standard propagation of the error in (kcat/KM) × Kop′ and kcat/KM.

e

Reported error is from standard propagation of the error in Kop′.