Table III.

Thermodynamic Parameters from the Alkaline Conformational Transition of WT and TM Iso-1-Cytochromes c at 22 ± 1°C and in 0.1M NaCl

Varianta	pKapp	nb
WT	8.00 ± 0.05	0.98 ± 0.01
TM	7.56 ± 0.05	1.00 ± 0.01
WT(C102T)c	7.95 ± 0.02	—

^aAll variants are expressed in E. coli, thus Lys 72 is not trimethylated.

^bn is the number of protons released in the alkaline conformational transition.

^cData are from Ref.27. Data were fit assuming n = 1.