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. 2009 Mar 30;18(6):1175–1182. doi: 10.1002/pro.123

Figure 4.

Figure 4

RhCTC binds to the TM region of (pro)SP-C. (A) binding of rhCTC to spots containing 10-residue fragments derived from SP-C (red part in Fig. 1). The peptide spots 1 to 25 cover the SP-C sequence in an N- to C-terminal direction. Spot 7 contains the KRLLIVVVVV segment in SP-C, whereas Spots 8 and 9 contain Leu- (KRLLLLLLLL) or Ala-substituted (KRAAAAAAAA) versions thereof, respectively. In the same manner spots 10–12 contain RLLIVVVVVV (positions 12–21 in SP-C), RLLLLLLLLL, and RAAAAAAAAA, respectively, and spots 16–18 contain VVVVVLVVVV (positions 17–26 in SP-C), LLLLLLLLLL, and AAAAAAAAAA, respectively. Spots 21 and 23 contain the same peptide (LVVVVIVGAL). (B) summary of rhCTC binding along the SP-C sequence. Dotted lines mark peptides to which rhCTC does not bind, while the solid lines represent peptides to which rhCTC binds. The underlined part of the SP-C sequence corresponds to the TM region. The numbering 1–35 refer to the mature SP-C peptide, the corresponding residues in proSP-C are 24–58 (see Fig. 1).