Figure 2.

Activity of ECU11_0510 against oligomeric N-acetyl glucosamine (NAG) in the presence or absence of metal ions. ECU11_0510 (4 μM) was assayed using a fluorescamine-based method for 16 h at 37°C with 2 mM of NAG substrates and 5 μM. Colletotrichum lindemuthianum chitin deacetylase (ClCDA, 500 nM) was used as a positive control and the assayed contained 2 mM NAG3 and 5 μM ZnCl. Experiments were performed in triplicate and standard deviations from the mean are shown as error bars.