Table II.
Cytotoxic Activity of the Immunotoxin B3-LLO
| EC50 (nM) |
||||
|---|---|---|---|---|
| Toxin | MCF7 (Lewis Y+) | SKBR-3 (Lewis Y+) | HeLa (Lewis Y−) | HT-29 (Lewis Y−) |
| E8C-LLOred | 1.9 ± 0.4 | 2.49 ± 0.15 | 10.34 ± 2.23 | 1.9 ± 0.25 |
| B3-LLO | 2.3 ± 0.5 | 12.7 ± 1.94 | 503.78 ± 164.11 | 168.9 ± 38.9 |
| E8C-LLOox | 153.8 ± 41 | 314.8 ± 40.67 | 910.63 ± 164.07 | 231.5 ± 58.1 |
EC50 values of the immunotoxin and isolated toxins in reduced (E8C-LLOred) and oxidized form (E8C-LLOox) in cell culture experiments using different antigen positive (Lewis Y+) and antigen negative (Lewis Y−) cell lines.