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Apparent Thermal Stabilities of the GFPN-EF1·EF2-GFPC and GFPN-ZN·ZC-GFPC Complexes at Different Calcium Concentrations. Apparent Tm-Values were Obtained by Fitting Eq. (1)to Normalized Fluorescence Data
| Condition | Tm (oC) GFPN-EF1·EF2-GFPC | ± (°C) | Tm (oC) GFPN-ZN·ZC-GFPC | ± (°C) |
|---|---|---|---|---|
| 1 mM EDTA | 75.3 | 0.10 | 75.3 | 0.16 |
| 10 μM Ca2+ | 78.0 | 0.13 | ||
| 100 μM Ca2+ | 78.1 | 0.16 | ||
| 1 mM Ca2+ | 78.1 | 0.16 | ||
| 10 mM Ca2+ | 78.2 | 0.14 | 75.6 | 0.12 |
