Table 1. Primers used for amplification of cDNA of the glutamate receptor isoforms, actin, rod-opsin, mGluR6, and melanopsin.
|
Amplicon |
Sequences |
bp |
| GluRl flip |
up: tgggtgcctttatgcagcaaggatg, low: gtccgtatggcttcattgatggattgc up: aatlgcttatggcacattggaagca. low: tcttgtccttacttccggagtcctt |
419 |
| GluRl flop |
up: tgggtgcctttatgcagcaaggatg. low: gtccgtatggcttcattgatggattgc up: aattgcttatggcacattggaagca. low: gtcttgtccttggagtcacctccccc |
419 |
| GluR2 flip |
up:: tgggtgcctttatgcagcaaggatg. low: gtaacagaactctatcaaagccacca up: ataaaatgtggacttatatgaggag. low: tcttgtccttacttccggagtcctt |
349 |
| GluR2 flop |
up: : tgggtgcctttatgcagcaaggatg. low: gtaacagaactctatcaaagccacca up: ataaaatgtggacttatatgaggag, low: gtcttgtccttggagtcacctccccc |
349 |
| GluRJ flip |
up: : tgggtgcctttatgcagcaaggatg, low: gtaacagaactctatcaaagccacca up: ccccatagagagcgctgaagatt. low: tcttetccttacttccggagtcctt |
458 |
| GluR3 flop |
up: : tgggtgcctttatgcagcaaggatg, low: gtaacagaactctatcaaagccacca up: ccccatagagagcgctgaagatt. low: gtctmtccttggagtcacctccccc |
458 |
| GluR4 flip |
up:: tgggtgcctttatgcagcaaggatg, low: gtaacagaactctatcaaagccacca up: attgcctatggaacacttgattcg. low: tcttgtccttacttccggagtcctt |
419 |
| GluR4 flop |
up: : tgggtgcctttatgcagcaaggatg. low: gtaacagaactctatcaaagccacca up: attgcctatggaacacttgattcg. low: gtcttgtccttggagtcacctccccc |
419 |
| KA1 |
up: ggctccaccatgaccttcttcca. low: tcccaccacttgcgcttcag
up: gaftgccagagtgttgaattccaat. low: atttccagccgnttgttctcctg |
207 |
| KA2 |
up: ggctccaccatgaccttcttcca. low: tcccaccacttgcgcttcag
up: aatcgcccgcgtcctcaactcccgc, low: atttccagccggttgttctcctg |
207 |
| GluR5 |
up: ccctgactcagacgtggtggaa, low: agaaggtcattgtcgagccatctc
up: gttggagctctcatgcagcaagg. low: agaaggtcattgtcgagccatctc |
358 |
| GluR6 |
up: ccctgactcagacgtggtggaa. low: ctatcttggtttgcttagctaaa
up: gttggagctctcatgcagcaagg. low: ctatcttggtttgcttagctaaa |
196 |
| GluR7 |
up: ccttacgagtggtatgatgctc. low: atgaaggcccacatcttctcaa
up: tctggtttggaatgggctccctg. low: atggtggccccgtccttgcacgca |
238 |
| delta 1 |
up: acagccaaccttgccgctttcctcac. low: ccataactctagagcagccacgagg up: tctggcggaccataagcaaga. low: cataactctagagcagccacgag |
439 |
| delta 2 |
up: acagccaaccttgccgctttcctcac. low: atcctctttcgatgggacccgggag up: caggatctgtccaagcaaacaga. low: ctcctcccttctgctttgcat |
469 |
| NR1(1) |
up: gctgtacctgctggaccgct. low: gcagtgtaggaagccactatgqatc |
219 |
| NR2a(1) |
up: gctacgggcagacagagaag. low: gtggttgtcatctggctcac |
257 |
| NR2b(2) |
up: gctacaacacccacgagaagag. low: gagagggtccacgctttcc
up: ccacgagaagaggatctaccag. low: caatgacaaacggtgcctcctc |
284 |
| NR2c(2) |
up: aaccacaccttcagcagcg. low: gacttcttgcccttggtgag.
up: atataaccccctacaccaagct. low: gttgtagctgacagggctgaaa |
416 |
| NR2d(1) |
up: cgatggcgtctggaatgg. low: agatgaaaactgtgacggcg |
265 |
| NR3a(1) |
up: ccgcgggatgccctactgttc. low: ccauttuttcatggtcaggat |
417 |
| actin(1) |
up: gggaaatcgtgcgtgacatt. low: cggatgtcaacgtcacactt |
255 |
| rod-opsin |
up: tacacactcaagcctgaggt. low: cctagtgggtgaauatgtatg |
291 |
| melanopsin |
up: tcttcatcttcagggccatc. low: ttctctgctgtaggccacat
up: tcaagagtccctgggttctg. low: ttagaccctgctacagatgtct |
362 |
| mGIuR6(3) | up: catggtcacgtgtacagtgtatgc. low: cttcttgaggctgcgcttccgc | 298 |
For most genes two sets of nested primers were used. The length of the amplicon in base pairs refers to the inner polymerase chain reaction product. The primers for the NMDA receptor subtypes were as described by Paarmann [24], with the exception of the inner primers for NR2b and NR2c. The primers for mGluR6 were as described by Maxeiner [25].