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IEF and oligosaccharide profiling analyses of recombinant acid α-glucosidase preparations. (a) IEF analysis of CHO-GAA (lane 2), HP-GAA (lane 3) and tgGAA (lane 4). Isoelectric point markers are shown in lane 1. (b) Oligosaccharide profiles of CHO-GAA, HP-GAA and tgGAA. Regions of the chromatogram containing neutral, sialylated (NANA), mannose-6-phosphate-containing (M6P) and bis mannose-6-phosphate-containing (Bis M6P) glycans are noted.
