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. Author manuscript; available in PMC: 2009 Nov 9.
Published in final edited form as: Sci Signal. 2009 May 5;2(69):ra20. doi: 10.1126/scisignal.1164302

Fig. 2.

Fig. 2

Time course of mediator binding to the Egr1 promoter in ES and MEF cells. ChIP for mediator at the Egr1 promoter in ES (white bars) and MEF (black bars) WT and KO cells in replicate. Sheared chromatin was immunoprecipitated with antibodies to both MED1 and MED17 to maximize the mediator ChIP signal. Samples were quantitated by real-time PCR and normalized to percent of signal relative to input chromatin. Egr1 intron PCRs at t = 0 for ES (left) and MEF cells (right) show the background, nonspecific signal from the ChIP. After 16 hours of serum-starvation, 20% FBS was added for 0, 5, 20, or 40 min before formaldehyde cross-linking. Error bars indicate SD from four independent experiments.