Figure 5.

Wnt reporter activity in CLL specimens. Bar diagram with wnt reporter activity. Each CLL sample was transfected with the negative control vector (neg K, firefly luciferase lacking TCF/LEF binding sites), or the wnt reporter (wnt rep, wnt reporter vector with TCF/LEF binding sites and empty vector), or the wnt reporter plus E-cadherin expression vector (wnt rep + E). Constitutively expressing Renilla luciferase was cotransfected in all of the groups as a control for transfection efficiency. Reporter expression of the negative control (firefly to Renilla ratio) was arbitrarily kept as 1 unit for each CLL case.