Fig. 3.

Effects of theanine on the migration of human lung cancer A549 cells and the anticancer activity of anticancer agents NCTD and BER against A549 cell migration. The cells were treated for 6 h with theanine (T) at the concentrations of 32–250 μM (T32, T64, T125, T250) in the absence or presence of norcantharidin (NCTD) at 2.5 μM and berbamine (BER) at 2.5 μM. The cells in the control group were treated with DMSO vehicle (0.1%, final concentration). The effects on the migration of A549 cells were examined by the migration assay as described in the section of “Materials and methods”. The data are presented as the mean ± SD (Bar) for each group (n = 6). The figure is the representative of three similar experiments performed. Comparison was made by two-way ANOVA followed by Bonferroni post hoc test to establish whether significant differences existed between the groups. Values with different letters (a–d) differ significantly (p < 0.05). d/e Represents the significant synergistic effects (T32 + NCTD2.5, p < 0.001, two-way ANOVA; T32 + BER2.5, p < 0.001, two-way ANOVA) compared with the treatment with its individual compound alone