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Inhibition by MC148P of chemokine-induced calcium mobilization. MC148P (amounts indicated to the right of each curve) and MCP-1 (1 nM) or I-309 (0.5 nM) were added successively to cells that had been loaded with Fura-2. Fluorescence was measured at 510 nm after repetitive, sequential excitations at 340 nm and 380 nm and the ratios were determined. (A) Primary monocytes. (B) CCR8-transfected murine pre B cells (12). (C) CCR2B-transfected HEK 293 cells (10).
