Fig. (4).

Effects of various RNAi oligos on neurotrophin-induced beta-lactamase reporter activity. Cells were incubated with indicated RNAi oligos for 32 hrs, followed by a medium change and additional 16 hour-incubation. Cells were then stimulated with respective ligands (NGF for TrkA cells, A; BDNF for TrkB cells, B; NT-3 for TrkC cells, C) for 5 hours before beta-lactamase assay was performed as described in Methods. Response Ratios were plotted for each RNAi oligo (n=3 for each data point). Unstim: untransfected cells were left unstimulated with ligands; Mock: cells were treated with transfection reagent (no RNAi oligo) and stimulated with ligands.