Fig. (5).

Effects of various RNAi oligos on thapsigargin-induced beta-lactamase reporter activity. TrkA-NFAT-bla CHO-K1 (A), TrkB-NFAT-bla CHO-K1 (B) and TrkC-NFAT-bla CHO-K1 (C) cells were incubated with indicated RNAi oligos for 32 hrs, followed by a medium change and additional 16 hour-incubation. Cells were then stimulated with thapsigargin for 5 hours before beta-lactamase assay was performed as described in Methods. Response Ratios were plotted for each RNAi oligo (n=3 for each data point). Unstim: untransfected cells were left unstimulated with ligands; Mock: cells were treated with transfection reagent (no RNAi oligo) and stimulated with thapsigargin.