Table 1.

Beta-Lactamase Assay Protocol for Trk-NFAT-bla CHO-K1 Cells in 384-Well Format

Step	Parameter	Value	Description
1	Cells	32 µL	Trk-NFAT-bla CHO-K1 cells, 10,000 cells/well
2	DMSO or Compound	4 µL	10 X compound solution or 1% DMSO
3	Neurotrophin	4 µL	10 X Neurotrophin
4	Incubation	5 hrs	37oC, 5% CO2
5	Substrate	8 µL	6 X CCF4–AM
6	Incubation	2 hrs	Room Temp.
7	Detection	fluorescence	Tecan Safire2