. 2008 Feb 25;1:34–42. doi: 10.2174/1875397300801010034

Table 1.

Summary Comparison of AMPK Isoform Properties. n.d. = Not Determined, n.a. = Not Applicable

		ATP Km (µM)		Inhibitor
AMPK Isoform	Assay Format	- AMP	+ AMP (100 µM)	IC₅₀(nM)	Activator EC₅₀(µM) / Max. fold-activation
AMPK Isoform	Assay Format	- AMP	+ AMP (100 µM)	Cpd C	AMP	ZMP	APR	ATMP
α₁β₁γ₁	Radiometric	34	74	230	1.6 / 1.7-fold	165 / 1.5-fold	n.d. / n.a.	0.7 / 1.7-fold
α₁β₁γ₁	TR-FRET	n.d.	n.d.	700	1.4 / n.a.	74 / n.a.	722 / n.a.	0.25 / n.a.
α₁β₁γ₁	FRET	35	83	320	2.5 / 2.8-fold	112 / 3.3-fold	n.d. / n.a.	0.35 / 3.3-fold
α₁β₁γ₁	CHEF	31	25	343	0.6 / 1.8-fold	36 / 1.8-fold	n.d. / n.a.	0.25 / 1.8-fold
α₂β₁γ₁	Radiometric	65	73	88	1.4 / 2.5-fold	54 / 2.5-fold	194 / 1.9-fold	0.13 / 2.9-fold
α₂β₁γ₁	TR-FRET	n.d.	n.d.	500	0.35 / n.a.	23 / n.a.	43 / n.a.	0.04 / n.a.
α₂β₁γ₁	FRET	165	154	140	1.4 / 5-fold	40 / 6.3-fold	n.d. / n.a.	0.10 / 6.3-fold
α₂β₁γ₁	CHEF	82	55	184	0.23 / 2.5-fold	40 / 2.2-fold	n.d. / n.a.	0.07 / 2.5-fold