Figure 3.
Multimerization of TRIM5αAGM is not modulated by infection with restriction-sensitive viruses. A, MDTF cells expressing TRIM5αAGM were either infected or not infected with HIV-1 for 6 hours, using a virus dose leading to about 15% infected cells and in the presence of 1 μM MG132. Crosslinking assays were done as before. B, MDTF-TRIM5αAGM cells were infected with identical amounts (as normalized by titration on parental cells) of B-MLV or N-MLV-derived vectors expressing GFP in the presence of MG132. 30% and 10% of the cells were infected (GFP-positive), respectively, by B-MLV-GFP and N-MLV-GFP, as seen by flow cytometry 2 days later.