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. 2008 May 23;1:54–64. doi: 10.2174/1875397300801010054

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© Adams et al.; Licensee Bentham Open.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. (5). Case Study 2: AKT dependent cSTKpep-3 phosphorylation. — (A) HEK293 cells were transiently double transfected (1µg kinase DNA and/or 1µg substrate DNA in 6-well dish) as indicated and immunoblotted. (B) 384-well TR-FRET assay using a transient reverse transfection protocol. Wild-type Akt1(WT) or kinase-inactive Akt1(K179M) were combined with cSTKpep-3.