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. 2008 May 23;1:54–64. doi: 10.2174/1875397300801010054

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© Adams et al.; Licensee Bentham Open.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. (6). Quantification of total- and phospho-cSTKpep levels can reveal compound interference. The importance of short compound incubation. — (A) Combinations of α-FLAG with α-p-STK or α-Myc antibodies were used to quantify either cSTKpep-phosphorylation or total cellular cSTKpep-3 expression by TR-FRET. (B) and (C) Transient 384-well AKT/cSTKpep-3 TR-FRET assay. Cells were treated with staurosporine for 2h (B) or 48h (C). Open circles: phospho-cSTKpep-3; Solid circles: total-cSTKpep-3.