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. 2009 Oct 29;9:129. doi: 10.1186/1471-2229-9-129

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Copyright © 2009 Lora et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mature pollen of Annona cherimola. (A) Intine (black arrow) is similar all around the pollen grain, but exine (white arrow) is thinner in the pollen aperture site. Longitudinal section stained with a 3:1 mixture of Auramine and Calcofluor. (B) Sibling pollen grains have a faint cohesion that showed with JIM 5 antibody the presence of unesterified pectins. (C) Mature pollen tetrad following acetolysis. (D, E, F) Mature pollen observed with scanning electron microscopy (SEM). (D) Mature pollen grains with a globose shape and a radiosymmetric disposition. (E) Exine cohesion helps keeping sibling pollen grains together. (F) Pollen exine shows a tectate perforate appearance. A, B, D: Bar = 20 μm; C: Bar = 10 μm; E, F: Bar = 2 μm.