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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Mol Cell. 2009 Oct 9;36(1):28–38. doi: 10.1016/j.molcel.2009.09.016

Figure 1. YOD1 associates with p97 via the N-terminal UBX domain.

Figure 1

(A) Domain organization of YOD1 and its mutant derivatives.

(B) 293T cells were transiently transfected with the indicated constructs or empty vector and homogenized by NP40 detergent lysis 24h post transfection. Lysates were subjected to immunoblotting with anti-FLAG and anti-p97 antibodies to control for expression of YOD1 derivatives and p97, respectively (upper and middle panels). Retrieved p97 in anti-FLAG immunoprecipitates was detected by immunoblotting with anti-p97 antibodies (lower panel).