Table 3-2.
Cardiovascular Proteins Regulated by S-Glutathionylation
| Protein | Citation | Function | Model system | Stimulus | Detection | Effect(s) | Reversed by GRx? |
|---|---|---|---|---|---|---|---|
| SERCA | 2 | cytosolic Ca2+ reuptake | SERCA isolated from rabbit heart; HEK cells w/transfected SERCA; aortic homogenates; aortic rings | In vitro: ONOO− + GSH-sepharose; ex vivo; ACh, NO, bradykinin | Affinity chromatography; Bio-GEE + 1D PAGE + anti-streptavidin; Mass Spec | ↑ Ca2+ uptake activity | Not shown |
| RyR | 265, 264 | SR Ca2+ release | SR vesicles from canine heart | Tachycardic pacing; NADPH + [35S]-GSH Exercise or tachycardia +/− I/R |
Tachycardia + 1D PAGE + anti-GSH Ab; phosphorimaging Tachycardia/Exercise + 1D PAGE + anti-GSH Ab |
↑ Ca2+ release rates (transient) ↑ Ca2+ release rates (transient); ↓ Ca2+ leak; ↓ infarct size |
Not shown Not shown |
| α-KGDH | 217 | TCA cycle | Rat heart mitochondria; purified αKGDH | H2O2; GSH/diamide | Reversibility by GRx1 | ↓ AKG dehydrogenase activity | via GRx1 |
| 8 | Cardiac mitochondria from male Sprague-Dawley rats | H2O2 | Reversibility by GRx1; NEM + GRx1 + Biotin-NEM; NEM + GRx1 + Biotin-SPDP | ↓ AKG dehydrogenase activity | via GRx1 |