Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Calcium. 2009 May 30;46(1):56–64. doi: 10.1016/j.ceca.2009.04.004

Figure 2. Inhibition of Plk1 activity by BI2536 decreases IP3R1 MPM-2 reactivity in GVBD oocytes.

Figure 2

(A) Immunoblotting of oocyte lysates collected at 0 hr (GV) or 2 hr (GVBD) and treated with the indicated concentrations of BI2536 (BI). The blot was probed with an MPM-2 antibody and after stripping re-probed with an anti-IP3R1 (Rbt03) antibody. (B) Quantification of IP3R1 MPM-2 reactivity. The intensity of the MPM-2 reactive band from GVBD oocytes was arbitrarily given the value of 1 and values in the other lanes were expressed relative to this band. (**, p<0.015)