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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Calcium. 2009 May 30;46(1):56–64. doi: 10.1016/j.ceca.2009.04.004

Figure 3. BI2536 reduces the increased IP3-induced Ca2+ release (IICR) in GVBD oocytes but does not affect the Ca2+ -store content.

Figure 3

The duration (A) and amplitude (B) of the Ca2+ rise was measured with Fluo-4 after releasing inositol 1,4,5-trisphosphate (IP3) by a UV-flash in GV and GVBD oocytes ± 10 μM BI2536. (C) A typical trace of IICR in GV, GVBD and GVBD oocytes treated with 10 μM BI2536. (D) The number of GV, GVBD and GVBD oocytes treated with 10 μM BI2536 that respond to IP3 with a Ca2+ rise. (E) The content of the Ca2+ stores in GV and GVBD oocytes ± 10 μM BI2536 was estimated by measuring the area under the curve of the Ca2+ response induced by the addition of thapsigargin (10 μM) in Ca2+ -free media. (*, p<0.03; **, p<0.015)