Figure 2. Synergy between ascr#2, ascr#3 and ascr#4.
a,Structures of ascr#1, ascr#2, ascr#3 and ascr#4. b, Dose-response curves of ascr#2 and ascr#3. c, Synergistic effects of ascr#2 and ascr#3 from points respectively indicated by red and blue arrows in b. d, Demonstration that the three synthetic compounds account for most of the mating activity. For all entries, ascr#4 was tested at 1 pmol and ascr#2 and ascr#3 were each tested at 20 fmol. n≥30 animals for each histogram. Error bars, s.e.m.; *P<0.01, ***P<0.0001, unpaired t-test.