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. Author manuscript; available in PMC: 2010 Jun 3.
Published in final edited form as: J Am Chem Soc. 2009 Jun 3;131(21):7293–7303. doi: 10.1021/ja805174z

Figure 4.

Figure 4

Uptake of 5-Fam labeled peptides in HeLa cells evaluated by fluorescence spectroscopy. Cells were treated with peptide at different concentrations (0, 0.1, 0.3, 1.0, 3.0 μM) for 1 h, washed, and lysed in RIPA buffer. The fluorescence of the lysates was then normalized against the amount of protein in the sample. Each experiment was performed in triplicate and the results are expressed as the mean relative fluorescence ± standard deviation.