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. Author manuscript; available in PMC: 2009 Nov 9.
Published in final edited form as: J Immunol. 2009 Jul 1;183(1):518–523. doi: 10.4049/jimmunol.0900068

FIGURE 1.

FIGURE 1

MZ and Fo B cells transferred into SCID mice generate TI Ab responses to PyV. A, MZ (B220+CD21highCD23low) and Fo (B220+ CD21lowCD23high) B cells were sorted from B6 splenocytes by FACS; the isolated populations are shown. B, VP1-specific ELISA of serum IgM and IgG of SCID mice reconstituted with 2 × 106 MZ B cells (n = 5), 2 × 106 Fo B cells (n = 5), or 1 × 107 Fo B cells (n = 9) and infected with PyV. The sera for IgM was taken on day 14 and for IgG on day 21 after infection and tested at 1/100 dilutions. Sera of uninfected B6 mice were used as negative controls. Mean absorbance at 450 nm with SEM are shown. Data are representative of three experiments.