Fig. 6.

Effects of CP mutants on reporter RNA translation. (a) Representative fluorescent microscopic examination of GFP expression from the 2GFP2 reporter in N. benthamiana leaves. Agrobacterium harboring 2GFP2 (at an OD₅₉₅ of 0.2) was co-infiltrated with the culture that can express either WT CP or the mutants (each at an OD₅₉₅ of 1.0). GFP images were taken at 40 hpi. (b) Western blot analysis of GFP and CP expression. The leaves were macerated, and approximately equal amounts of protein lysates were subjected to SDS-PAGE followed by Western blot with either rabbit anti-CP polyclonal antibody or mouse anti-GFP monoclonal antibody. The secondary antibodies used were a goat anti-rabbit or a goat anti-mouse. “Rubisco” denotes the small subunit of the Rubisco protein that served as a protein loading control.