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. Author manuscript; available in PMC: 2009 Nov 9.
Published in final edited form as: Neuroscience. 2007 Nov 12;151(1):303–312. doi: 10.1016/j.neuroscience.2007.10.037

Fig. 2.

Fig. 2

Submillimolar concentrations of pilocarpine induce muscarine-receptor dependent fast activity in the hippocampus of the in vitro isolated guinea-pig brain. In the upper panel, the positions of the recording electrodes in the isolated brain are illustrated: PC, EC and hippocampus (CA1 area). (A) Reversible effect of arterial perfusion of pilocarpine 100 μM in PC, EC and CA1. (B) Fast activity in CA1 region induced by different concentrations of pilocarpine (100 and 800 μM) diluted in the arterial perfusate (middle traces). Field activity before and 20 min after pilocarpine perfusion is illustrated in the upper and lower traces. (C) The effect of pilocarpine observed in CA1 is abolished by co-perfusion of the isolated brain with pilocarpine and atropine sulfate (5 μM). (D) Mean frequency power of CA1 fast activity recorded during application of 800 μM pilocarpine (n=8; continuous thick line), co-application of 800 μM pilocarpine and atropine 5 μM (n=4; thin continuous line) and after drug washout (n=8; dotted line).