Skip to main content
. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Dec 27;484(2):155–166. doi: 10.1016/j.abb.2008.12.012

Figure 3.

Figure 3

Sequence-specific identification of tyrosine nitration in rabbit muscle phosphorylase b (15 µM) incubated with 1 mM peroxynitrite in vitro. A – Total ion current chromatogram showing retention times for selected native and respective nitropeptides. B - Identification of nitrated Tyr residues by tandem MS for a representative 3-NT containing peptide, VLY280(NO2)PNDNFFEGK. The insert illustrates the use of a ladder of sequence-indicating fragments from N- (b) and C- (y”) termini for the identification of the peptide sequence.