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. Author manuscript; available in PMC: 2009 Nov 9.
Published in final edited form as: Cancer Res. 2007 Jun 1;67(11):5505–5512. doi: 10.1158/0008-5472.CAN-06-3647

Figure 3.

Figure 3

PELP1 promotes cytoskeletal reorganization and motility. A, Western blot analysis of PELP1 expression in lysates from cells derived from the MCF10AT model system. MCF10A, nonmalignant human breast cancer cells; MCF10-AT, weakly tumorigenic; MCF10-DCIS, comedo-type DCIS, highly proliferative, aggressive, and invasive; and MCF10-CA, undifferentiated carcinomas, metastatic. B, total cell lysates from pcDNA- and PELP1-expressing clones (nos. 13, 20, Pool) were analyzed by Western blotting with an anti-PELP1 antibody (left). Actin was used as a loading control. The functionality of PELP1-siRNA was analyzed by Western blotting (right). C, migratory potential of MCF-7 clones overexpressing PELP1 was analyzed by Boyden chamber assay (top). MCF-7 cells stably overexpressing PELP1 and MCF-7 cells transfected with PELP1-siRNA were treated with E2 or were untreated, and the cell migration potential was analyzed by means of the Boyden chamber assay (bottom). D, MCF-7, MCF-7–PELP1 clone nos. 13 and 20 were cultured in a DCC-serum–containing medium, after which they were treated with E2 for 10 min. The status of filamentous actin was visualized by phalloidin staining and was evaluated by fluorescence microscopy.