Table 2.
Primers for human DISP1 mutational analysis
Exon | Primer | T(°C) PCR | Size (bp) | Tm(°C) dHPLC |
---|---|---|---|---|
1 | 5′ TCTTACTTAGAGTCAAGAAATTGG 3 | 53 | 509 | 60.5 |
5′ TGAATGCTAAAAGCAAAACTTTCG 3′ | ||||
2 | 5′ ATGTTATGATGTTTATGATGCTCTG 3′ | 50 | 250 | 54 |
5′ GAATTCCTCAAGCAGCCAACTCATG 3′ | ||||
3 | 5′ AGTTATGCAGCTCTGATAGCCGAC 3′ | 54 | 216 | 59 |
5′ CAATATTTGGAGATGATTTTAGGC 3′ | ||||
4–5 | 5′ ACTAATGAGCACCTGTAATTTTGC 3′ | 51 | 648 | 55 |
5′ TGGTTTGTTCATCTACAATGTCAC 3′ | ||||
6 | 5′ TGAATTATTTCCAAATCCTGAGTC 3′ | 45 | 233 | 53 |
5′ TAATACAACCTTATTTGTGCTAAG 3′ | ||||
7A | 5′ CCTTCTGCTTGTCTCTATCTCTGC 3′ | 53 | 525 | 58 |
5′ AGTATCCATTAGAAGATAATCCTG 3′ | ||||
7B | 5′ GATTGAGTTTGGTATCAAACACAG 3′ | 53 | 541 | 57.5 |
5′ TATATTAAGAAGATACCGCTCATG 3′ | ||||
7C | 5′ CATGGCTTCCAGCAGTTGTTGTGC 3′ | 53 | 565 | 58 |
5′ GTTTGGTTTCTCAGTTTTTGACAG 3′ | ||||
7D | 5′ ACATCGCCAGCCCAGCTTCCCAGG 3′ | 58 | 563 | 57.5 |
5′ CCAAGTTGTCAGCAGCATCACGCT 3′ | ||||
7E | 5′ CATGGGGCTGTCAGTTGCTGTTGC 3′ | 58 | 556 | 59 |
5′ GTATGTGTTTTGCTTTGTCCCTTG 3′ | ||||
7F | 5′ TACAGTGCAGTGCCTTTTCCCATG 3′ | 58 | 568 | 59 |
5′ AGGCAGGGACAGTGGTGGATGTGC 3′ | ||||
7G | 5′ TGAAGGCCACACACCAAGCTGTCG 3′ | 58 | 632 | 59 |
5′ AGGCACTGGTTCTGAATTGAATGC 3′ |