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In situ hybridizations are shown for +/+ (left) versus N629D/N629D embryos (right) at E9.5. Substantial Hand2 expression is observed in the branchial arches (BA), outflow tract (OT), right ventricle (RV), with less expression in the left ventricle (LV). Hand2 expression is downregulated in N629D/N629D embryos in these similar locations (right Panel). Bar indicate 200 µM.
