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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Am J Surg. 2009 Nov;198(5):611–616. doi: 10.1016/j.amjsurg.2009.07.006

Figure 1. Pressure effect on phagocytosis.

Figure 1

Figure 1

A: Pressure effect on phagocytosis by GD25-β1A null fibroblast cells. Null cells were incubated with serum-opsonized fluorescent-labeled latex beads under ambient pressure, or 20 mmHg increased pressure conditions for 2 hours, non-adherent beads were washed away, the cells were fixed, and the number of intracellular latex beads was counted. Data were expressed as percent phagocytosis as detailed in the Methods section. Pressure did not affect phagocytosis by GD25-β1A null fibroblast cells.

B: Pressure effect on GD25-β1A WT fibroblast cells phagocytosis. GD25-β1A WT fibroblasts were incubated with serum-opsonized fluorescent-labeled latex beads under ambient pressure, or 20 mmHg increased pressure conditions for 2 hours, non-adherent beads were washed away, the cells were fixed, and the number of intracellular latex beads was counted. Data were expressed as percent phagocytosis as detailed in the Methods section. Pressure increased GD25-β1A WT fibroblast (* p<0.01, n=6).