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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Am J Surg. 2009 Nov;198(5):611–616. doi: 10.1016/j.amjsurg.2009.07.006

Figure 3. Pressure effect on phagocytosis in cells expressing point-mutated b1 integrin constructs.

Figure 3

Pressure effect on phagocytosis by GD25 fibroblasts expressing wild type (WT) β1A subunit compared to fibroblasts expressing three different point mutated constructs: GD25-β1A T788A, GD25-β1A T788/9AA, GD25-β1A S785A. All cell lines were incubated with serum-opsonized fluorescent-labeled latex beads under ambient pressure (open bars), or 20 mmHg increased pressure conditions (shaded bars) for 2 hours, non-adherent beads were washed away, the cells were fixed, and the number of intracellular latex beads was counted. Data were expressed as percent phagocytosis as detailed in the Methods section Pressure increased phagocytosis in GD25-β1A WT fibroblast (* p<0.01, n=6), GD25-β1A T788D (* p<0.01, n=6) fibroblast and GD25-β1A S785A (* p<0.01, n=6) fibroblast cells but did not increase in GD25-β1A T788/9AA fibroblasts.