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. Author manuscript; available in PMC: 2010 May 5.
Published in final edited form as: Nature. 2009 Nov 5;462(7269):58–64. doi: 10.1038/nature08497

Figure 5. ERα-bound chromatin interactions are required for transcription activation.

Figure 5

Genome browser at the GREB1 locus showing data tracks: H3K4me3 and RNAPII ChIP-Seq (1 and 2); RNAPII ChIP-qPCR scans (3 and 4) using different RNAPII antibodies under oestrogen-induction (E2, in red) and ethanol control (ET, in grey); ERα (orange) and FoxA1 (green) ChIP-chip7 (5); ChIA-PET density (6) and interaction data (7). Inset: siRNA knockdown experiments. MCF-7 cells were transfected with siRNA against ERα (siERα) or control (siCtrl), and then analyzed by (a) western blot with ERα and calnexin (control) antibodies; (b) RT-qPCR on GREB1 expression; and (c) 3C assays at GREB1: siERα knockdown abolishes chromatin interactions and turns off transcription.