Figure 6. Double-staining of keratin-14 and involucrin in 21-day skin equivalents.

In Ker-CT keratinocytes keratin-14 (red) stained the lowermost layers, while involucrin (green) stained the uppermost layers. There was some evidence of co-localization (yellow to orange) in the intermediate layers. Upper staining of involucrin and lower staining of keratin-14 was partially retained in Ker-CT-Ras keratinocytes, but less so in Ker-CT-Ras-p53 keratinocytes. In Ker-CT-Ras-T keratinocytes there was little or no organization of the respective stains. In all tested cells, however, cellular co-localization of both proteins was rare. Lower images are insets of corresponding upper images. Scale bar: 40 µm.