Figure 9. Combined analysis of protein overexpression effects on epithelial integrity.

CDK4 and hTERT expression in keratinocytes demonstrate little or no effect to overall tissue integrity or cellular protein staining profile. Upon expression of H-Ras the tissue integrity is affected but the cellular staining profile remains similar to cells without H-Ras expression. After p53 inhibition and transformation, both tissue integrity and cellular identity is lost. Tissue integrity loss may be due to the inappropriate environment itself.