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. 2009 Nov 18;4(11):e7833. doi: 10.1371/journal.pone.0007833

Figure 4. Targeted knockdown of MRP leads to establishment of adherens junctions.

Figure 4

a) Bright field imaging of EpRas, C1, E13, and E14 at 10X. b) EpRas, C1, E13 or E14 cells were stained with primary antibodies specific to either E-cadherin (Becton Dickinson), APC (Calbiochem) or β-catenin (Becton Dickinson) and secondary antibodies Alexa fluor 488 anti-rabbit or anti-mouse (Invitrogen). Images at 100X c) Western blot analysis of EpRas, C1, E13, E14 whole cell lysates and immunoblotted with antibodies to Ras (Cell Signaling), E-cadherin and β-catenin (Becton Dickinson), and γ-tubulin (Sigma). NS =  non-specific. d) Indicated cells transfected with the TCF luciferase reporter construct TOPFLASH and transfection control pRL-CMV were treated with 0, 50 ng/mL or 100 ng/mL of recombinant WNT3a for 18 hours. All data is mean of three independent experiments ± SD.